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  5. Egg culture, sperm, preparation, and fertilization

Inseminated oocyte
This fluid, the culture medium, contains very precisely measured amounts of chemicals needed for the egg's survival.

Before the egg is incubated, usually shortly before egg collection, we ask the husband to produce semen by masturbation. The semen is taken to the laboratory. In the laboratory, the sperm are washed and diluted and the number of sperm counted under a microscope. Several hours after egg collection, spermatozoa will be mixed with the fluid containing the egg. Hopefully, fertilization will take place within a few hours. It is not uncommon to find a problem with the sperm - which may be too few in number or too weak in other ways. At present we need several thousand normal sperm for standard/conditional/routine IVF otherwhise we proceed to sperm micro injection (ICSI).

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